Coupling of Reduced Pyridine Nucleotide in Leuconostoc mesenteroides

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Coupling of Reduced Pyridine Nucleotide in Leuconostoc Mesenteroides.

The demonstration that oxidation-reduction reactions mediated by the pyridine nucleotide coenzymes occur with the transfer of hydrogen between substrate and coenzyme (1) has led to the use of hydrogen isotopes to measure the occurrence of the specific coupling of reactions in the intact cell and organism (2-4). Several problems associated with the cellular organization and integration of the fe...

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A reduced pyridine nucleotide pyrophosphatase.

Reaction 1 is catalyzed by DPNase and has been observed in higher animals (1, 2), in Neurospora (3), and in some bacteria (4). Reaction 2 is catalyzed by a deaminase found in Taka-Diastase (5). Reaction 3 is the DPN pyrophosphorylase from hog liver, as described by Kornberg (6) ; it is the only reversible reaction of those shown. The pyrophosphatase splitting shown in Reaction 4 has been observ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1964

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)93844-5